How are glass bottom cell culture dishes typically used?

1. Maintain sterility: Open dishes in a sterile environment (e.g. laminar flow hood).

2. Pre-equilibrate dishes: Incubate the dishes with culture medium. Pipet 2-3 ml of medium into the 35 mm glass bottom cell culture dishes or 3-4 ml into the 50 mm glass bottom cell culture dishes and incubate at 37° C for 15 minutes.

3. Add cell suspension to microwell: Remove the culture medium by aspiration and plate cells onto the glass surface. Pipet 250μl of the cell suspension (cells suspended in culture medium) into the 10 mm diameter microwells or 500μl of cell suspension into the 14 mm microwells. Incubate the dishes for 1 hour at 37° C.

4. Add additional medium: After 1 hour, gently fill the remainder of the dish with medium Add 2-3 ml to the 35 mm glass bottom cell culture dishes or 3-4 ml for the 50 mm glass bottom cell culture dishes. Note: After the initial one hour period to allow cells to attach to the glass surface, it is important to fill the dish to normal levels in order to minimize the effects of evaporation and to avoid inducing changes in osmolarity.

Biousing 35mm Glass Bottom Cell Culture Dishes Product Features:

Glass bottom dishes have a thin and optically clear bottom. A clinically non-toxic adhesive is used to attach the cover glass to the bottom of the TC treated culture dish. Round cover glass is used to make this product more elaborate.