Peter Chan’s Blog

Peter Chan’s IT blog, focus on IT Technology, news, ccna, ccnp…

Selected Way to Polish Sliver

Christmas Day is coming, tiffany jewelry is not a bad idea for a christmas gift. Tiffany Mall provides various kind of discount tiffany jewelry, like tiffany rings and tiffany accessories. They all made by sliver, so it’s much more cheaper than those made by gold, pt or diamond, but they’re designed by prestigious designers, that’s […]

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CCIE Voice Information

The Voice track concentrates on voice solutions for the enterprise and includes subjects such as QoS, MGCP, Call Manager (Cisco’s VoIP PBX), Cisco Unity (Cisco’s Unified Messaging platform), Unity Express and IP Contact Center Express.The CCIE Voice (CCIE Voice Written Exam (305-030) & CCIE Voice Lab Exam) certification is said to […]

Biousing Centrifuge Tubes

Centrifuge tubes or centrifuge tips are tapered tubes of various sizes made of glass or plastic. They may vary in capacity from tens of millilitres, to much smaller capacities used in microcentrifuges used extensively in molecular biology laboratories. The most commonly encountered tubes are of about the size and shape of a normal test tube […]

Cryopreservation of Cell Lines

The aim of cryopreservation is to enable stocks of cells to be stored to prevent the need to have all cell lines in culture at all times. It is invaluable when dealing with cells of limited life span. The other main advantages of cryopreservation are:

Reduced risk of microbial contamination
Reduced risk of cross contamination with other […]

Sourcing of Cell Lines

Sourcing of Cell Lines
Large numbers of cell lines look identical. Cell lines with very different origins and biological characteristics typically cannot be separated on grounds of morphology or culture characteristics. Infection or contamination of a cell line with an adventitious virus or mycoplasma may significantly change the characteristics of the cells but again such contamination […]

Resuscitation of Frozen Cell Lines

Many cultures obtained from a culture collection, such as ECACC, will arrive frozen and in order to use them the cells must be thawed and put into culture. It is vital to thaw cells correctly in order to maintain the viability of the culture and enable the culture to recover more quickly. Some cryoprotectants, such […]

Biousing Cell Culture Dishes List

 Cell culture is the process by which prokaryotic, eukaryotic or plant cells are grown under controlled conditions. In practice the term “cell culture” has come to refer to the culturing of cells derived from multicellular eukaryotes, especially animal cells. The historical development and methods of cell culture are closely interrelated to those of tissue culture […]

Introduction of 96 Well ELISA Plate

A Microtiter plate (spelt Microtitre in Europe) or microplate is a flat plate with multiple “wells” used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing […]

 How are glass bottom cell culture dishes typically used?
1. Maintain sterility: Open dishes in a sterile environment (e.g. laminar flow hood).
2. Pre-equilibrate dishes: Incubate the dishes with culture medium. Pipet 2-3 ml of medium into the 35 mm glass bottom cell culture dishes or 3-4 ml into the 50 mm glass bottom cell culture dishes […]

Trying to appease customers who ended up on the losing side of the recent high-definition movie format war, Best Buy on Wednesday said it would hand out $50 gift cards to buyers of HD DVD players. By surrendering to Sony-backed Blu-ray last month, Toshiba-led proponents of HD DVD left player owners holding devices reduced […]

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